SS-31

Cardiolipin-binding mitochondrial peptide

Technical datasheetLZH-004

Code: Elamipretide
Formula: C32H49N9O5
Molecular weight: 639.8 Da
Half-life: ~2 h (plasma); mitochondrial residence much longer
Research route: Subcutaneous / IV (research)
Molecular targets: Cardiolipin (inner mitochondrial membrane)
Discovery / disclosure: Szeto-Schiller laboratory, Cornell, ca. 2004
Target purity: ≥ 98%
Recommended storage: −20°C, light protected. 2 to 8°C after reconstitution.

Aliases

Elamipretide, MTP-131, Bendavia

Appearance

White lyophilized powder

Solubility

Freely soluble in water

Sequence

D-Arg-Dmt-Lys-Phe-NH2

Analysts reviewing the HPLC chromatogram for the current batch — FIG. 201Analytical Lab
Identity, purity and main-peak area are checked by reversed-phase HPLC against an in-house reference standard for this sequence. The chromatogram you see on the certificate of analysis is generated at this exact bench, by the analysts who release the batch.

Technician monitoring a stainless steel reactor during scale-up — FIG. 202Scale-up Operations
When a sequence moves from milligram research lots to multi-gram research supply, the same chemistry is transferred onto jacketed stainless reactors. Temperature, agitation and reagent equivalents are logged in real time so that the larger batch matches the analytical fingerprint of the small one.

Overview

SS-31 (elamipretide) is a tetrapeptide that selectively binds cardiolipin on the inner mitochondrial membrane, a key tool molecule in mitochondrial biology research.

Pharmacology and mechanism

Binding cardiolipin stabilizes the electron transport chain complexes, reducing electron leak and reactive oxygen species, restoring ATP synthesis efficiency.